Adult human neural cells in culture following traumatic brain injury

Objective: The present study aims to evaluate the viability of adult human neural cells in culture obtained from traumatized brain tissues collected in emergency surgery procedures. Methods: Exploratory, descriptive, quantitative and cross-sectional study evaluating samples obtained from patients who underwent traumatic brain injury with extrusion of brain tissue submitted to cell culture in a standardized medium, being preserved during 168h. After observation under phase contrast microscopy and immunohistochemical processing for neuronal (MAP-2) and glial (GFAP) markers, morphometric parameters of neural cells (cell body area, dendritic field length and fractal dimension) were evaluated using ImageJ software, with data obtained after 24, 72 and 168h being compared using non-parametric Kruskal Wallis test, followed by Dunn's post hoc test. Results: The explant of the nervous tissue revealed a consolidated pattern of cell migration into the culture medium. Cell proliferation, upon reaching confluence, presented an aspect of cellular distribution juxtaposed along the culture medium at all time points analyzed. Both neurons and glial cells remained viable after 168h in culture, with their morphologies not varying significantly throughout the time points evaluated. Immunohistochemistry for MAP-2 showed a relatively well-preserved cytoskeletal organization. GFAP immunoreactivity revealed activated astrocytes especially at the later time point. Conclusions: Our results point out the viability of cell culture from traumatized human nervous tissue, opening up perspectives for the use of substances of natural origin that may contribute neuroprotectively to neuronal maintenance in culture, allowing future translational approach.

Análise do teor de cloro ativo e ph de soluções de hipoclorito de sódio / Analysis of the active chlorine content and ph of sodium hypochlorite solutions

O objetivo do presente estudo é verificar o teor de cloro ativo e pH das soluções de hipoclorito de sódio nas concentrações de 0,5%, 1% e 2,5% adquiridas em lojas de material odontológico de Teresina - PI. Analisou-se 11 amostras medindo o teor de cloro ativo através do método da titulometria e pH utilizando pHmetro eletrônico. Observou-se que as substâncias analisadas se encontravam com o teor de cloro acima do especificado nos rótulos e pH alcalino, sendo apenas uma das amostras com o teor abaixo do especificado e pH menor que 9. De acordo com os resultados, conclui-se que as amostras avaliadas com soluções de hipoclorito de sódio dotam de concentrações de cloro diferentes daquelas especificadas nos rótulos.

One of the most important stages of endodontic treatment is the chemical-mechanical preparation that aims to generate cleaning, with the use of chemical irrigation solutions and instruments that help to sanitize the canal. The objective of the present study is to verify the active chlorine content and pH of the sodium hypochlorite solutions at the concentrations of 0.5%, 1% and 2.5% obtained at dental material stores in Teresina - PI. The 11 samples were analyzed by measuring the active chlorine content using the titration method and pH using electronic pH meter. It was observed that the substances analyzed were chlorine content higher than specified on the labels and alkaline pH, being only one of the samples with chlorine content below the specified and pH less than 9. According to the results, it was concluded that samples tested with sodium hypochlorite solutions were contained chlorine concentrations different from those specified on the labels.